The DS-160 form itself does not require a fee at the time of submission. Instead, you pay the non-refundable Machine-Readable Visa (MRV) fee when scheduling your visa appointment through the U.S. Visa Information and Appointment Services website. Payment methods vary by country but may include online banking, debit/credit card, or cash at designated banks. Once paid, you will receive a receipt with a reference number, which is required to proceed with booking your interview. It is essential to keep the receipt safely, as proof of payment is mandatory at the time of your appointment.